Embryonic stem cell-specific microRNAs promote induced pluripotency

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This paper reports my first exploration into the use of microRNAs to induce transitions in cells state. Using the Yamanaka-strategy, I asked whether miRNAs that were highly and specifically expressed in ESCs could aid in the reprogramming of differentiated cells into iPSCs. We found that miRNAs from the miR-302 and miR-290 clusters could replace cMyc in the Oct4/Sox2/Klf4/cMyc cocktail of transcription factors and induce full de-differentiation of mouse fibroblasts. Interestingly, the microRNAs also conferred more specificity to the transition as compared to cMyc, and did not result in partially reprogrammed colonies or transformed cells.

miR-294 and miR-302 increase the sensitivity of fibroblasts to OSK-induced somatic cell reprogramming. Point mutations to the seed sequence abrogate this effect. Increasing doses of miR-294 bring the efficiency to Myc-like levels.

Publications

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Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi.

Nature Communications accepted 2017

Iwei Yeh, Ursula Lang, Emeline Durieux, Meng Kian Tee, Aparna Jorapur, Alan Shain, Veronique Haddad, Daniel Pissaloux, Xu Chen, Lorenzo C...

High accuracy label-free classification of single cell kinetic states from holographic cytometry of human melanoma cells.

Scientific Reports accepted 2017

Miro Hejna, Aparna Jorpaur, Jun Song, Robert L Judson.

CDK1 Inhibition Targets the p53-NOXA-MCL1 Axis, Selectively Kills Embryonic Stem Cells, and Prevents Teratoma Formation

Stem Cell Reports 2015

Noelle E Huskey, Tingxia Guo, Kimberley J Evason, Olga Momcilovic, David Pardo, Katelyn J Creasman, Robert L Judson, Robert Blelloch, Sco...

Two miRNA Clusters Reveal Alternative Paths in Late-Stage Reprogramming

Cell Stem Cell 2014

Ronald J Parchem, Julia Ye, Robert L Judson, Marie F Larussa,Raga Krishnakumar, Amy Blelloch, Michael C Oldham, Robert Blelloch

MicroRNA-based discovery of barriers to dedifferentiation of fibroblasts to pluripotent stem cells

Nature Structural & Molecular Biology 2013

Robert L Judson, Tobias S Greve, Ronald J Parchem, Robert Blelloch

microRNA Control of Mouse and Human Pluripotent Stem Cell Behavior

Annual Review of Cell and Developmental Biology 2013

Tobias S Greve, Robert L Judson, Robert Blelloch

Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells

Nature Biotechnology 2011

Deepa Subramanyam, Samy Lamouille, Robert L Judson, Jason Y Liu, Nathan Bucay, Rik Derynck, Robert Blelloch

miR-380-5p represses p53 to control cellular survival and is associated with poor outcome in MYCN-amplified neuroblastoma

Nature Medicine 2010

Alexander Swarbrick, Susan L Woods, Alexander Shaw, Asha Balakrishnan, Yuwei Phua, Akira Nguyen, Yvan Chanthery, Lionel Lim, Lesley J Ash...

Opposing microRNA families regulate self-renewal in mouse embryonic stem cells

Nature 2010

Collin Melton, Robert L Judson, Robert Blelloch

Embryonic stem cell-specific microRNAs promote induced pluripotency

Nature Biotechnology 2009

Robert L Judson, Joshua E Babiarz, Monica Venere, Robert Blelloch

The GP(Y/F) domain of TF1 integrase multimerizes when present in a fragment, and substitutions in this domain reduce enzymatic activity of the full-length protein

Journal of Biological Chemistry 2008

Hirotaka Ebina, Atreyi Ghatak Chatterjee, Robert L Judson, Henry L Levin

The self primer of the long terminal repeat retrotransposon Tf1 is not removed during reverse transcription.

Journal of Virology 2006

Angela Atwood-Moore, Kenneth Yan, Robert L Judson, Henry L Levin

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