Normal (wild type) or microRNA-null (DGCR8 -/-) ESCs with different combinations of individual miRNAs re-introduced and then stained for a marker of pluripotency.

Opposing microRNA families regulate self-renewal in mouse embryonic stem cells

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Collin Melton performed screens for microRNAs that caused ESCs to differentiate. He found that the miRNA family, Let-7, induced differentiation in ESCs that lacked all endogenous miRNAs, but not in wild type ESCs. His conclusion - there must be an endogenous miRNA family that stabilizes the pluripotency program against Let-7-induced differentiation. He went on to identify this family (the miR-294/302 family), and to identify a network of Let-7-regulated genes, a network of miR-294-regulated genes, and even a set of genes inversely regulated by the two families. We also found that endogenously expressed Let-7 stabilized differentiated cells against re-acquiring the pluripotency program. In short, two conserved miRNA families, each stabilizing the programs of the cell type in which they are normally expressed, and each destabilizing the programs of cell types in which they are normally silenced.

Publications

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Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi.

Nature Communications accepted 2017

Iwei Yeh, Ursula Lang, Emeline Durieux, Meng Kian Tee, Aparna Jorapur, Alan Shain, Veronique Haddad, Daniel Pissaloux, Xu Chen, Lorenzo C...

High accuracy label-free classification of single cell kinetic states from holographic cytometry of human melanoma cells.

Scientific Reports accepted 2017

Miro Hejna, Aparna Jorpaur, Jun Song, Robert L Judson.

CDK1 Inhibition Targets the p53-NOXA-MCL1 Axis, Selectively Kills Embryonic Stem Cells, and Prevents Teratoma Formation

Stem Cell Reports 2015

Noelle E Huskey, Tingxia Guo, Kimberley J Evason, Olga Momcilovic, David Pardo, Katelyn J Creasman, Robert L Judson, Robert Blelloch, Sco...

Two miRNA Clusters Reveal Alternative Paths in Late-Stage Reprogramming

Cell Stem Cell 2014

Ronald J Parchem, Julia Ye, Robert L Judson, Marie F Larussa,Raga Krishnakumar, Amy Blelloch, Michael C Oldham, Robert Blelloch

MicroRNA-based discovery of barriers to dedifferentiation of fibroblasts to pluripotent stem cells

Nature Structural & Molecular Biology 2013

Robert L Judson, Tobias S Greve, Ronald J Parchem, Robert Blelloch

microRNA Control of Mouse and Human Pluripotent Stem Cell Behavior

Annual Review of Cell and Developmental Biology 2013

Tobias S Greve, Robert L Judson, Robert Blelloch

Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells

Nature Biotechnology 2011

Deepa Subramanyam, Samy Lamouille, Robert L Judson, Jason Y Liu, Nathan Bucay, Rik Derynck, Robert Blelloch

miR-380-5p represses p53 to control cellular survival and is associated with poor outcome in MYCN-amplified neuroblastoma

Nature Medicine 2010

Alexander Swarbrick, Susan L Woods, Alexander Shaw, Asha Balakrishnan, Yuwei Phua, Akira Nguyen, Yvan Chanthery, Lionel Lim, Lesley J Ash...

Opposing microRNA families regulate self-renewal in mouse embryonic stem cells

Nature 2010

Collin Melton, Robert L Judson, Robert Blelloch

Embryonic stem cell-specific microRNAs promote induced pluripotency

Nature Biotechnology 2009

Robert L Judson, Joshua E Babiarz, Monica Venere, Robert Blelloch

The GP(Y/F) domain of TF1 integrase multimerizes when present in a fragment, and substitutions in this domain reduce enzymatic activity of the full-length protein

Journal of Biological Chemistry 2008

Hirotaka Ebina, Atreyi Ghatak Chatterjee, Robert L Judson, Henry L Levin

The self primer of the long terminal repeat retrotransposon Tf1 is not removed during reverse transcription.

Journal of Virology 2006

Angela Atwood-Moore, Kenneth Yan, Robert L Judson, Henry L Levin

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