miR-294/372 and miR-302 down-regulate TGF-beta receptor 2, TGF-beta signaling, and the TGF-beta induced epithelial to mesenchymal transition.
Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells
Published 2011 in Nature Biotechnology
Deepa Subramanyam, Samy Lamouille, Robert L Judson, Jason Y Liu, Nathan Bucay, Rik Derynck, Robert Blelloch
This paper was the result of a fun and fruitful collaboration between the Blelloch and Derynck labs. Led by Deepa Subramanyam (who now runs her own lab), the team translated our previous findings regarding the reprogramming potential of miR-302 from mouse to human cells, then delves into the mechanism for the first time by both identifying a handful of new targets, and demonstrating that miR-302 is a potent inhibitor of TGF-Beta signaling, which, in turn regulates transitions between mesenchymal and epithelial cell states. I had the opportunity to help spearhead the TGF-Beta experiments with Samy Lamouille from Rik’s lab, and learned a great deal in the process.