miR-294/372 and miR-302 down-regulate TGF-beta receptor 2, TGF-beta signaling, and the TGF-beta induced epithelial to mesenchymal transition.

Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells

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This paper was the result of a fun and fruitful collaboration between the Blelloch and Derynck labs. Led by Deepa Subramanyam (who now runs her own lab), the team translated our previous findings regarding the reprogramming potential of miR-302 from mouse to human cells, then delves into the mechanism for the first time by both identifying a handful of new targets, and demonstrating that miR-302 is a potent inhibitor of TGF-Beta signaling, which, in turn regulates transitions between mesenchymal and epithelial cell states. I had the opportunity to help spearhead the TGF-Beta experiments with Samy Lamouille from Rik’s lab, and learned a great deal in the process.

Publications

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Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi.

Nature Communications accepted 2017

Iwei Yeh, Ursula Lang, Emeline Durieux, Meng Kian Tee, Aparna Jorapur, Alan Shain, Veronique Haddad, Daniel Pissaloux, Xu Chen, Lorenzo C...

High accuracy label-free classification of single cell kinetic states from holographic cytometry of human melanoma cells.

Scientific Reports accepted 2017

Miro Hejna, Aparna Jorpaur, Jun Song, Robert L Judson.

CDK1 Inhibition Targets the p53-NOXA-MCL1 Axis, Selectively Kills Embryonic Stem Cells, and Prevents Teratoma Formation

Stem Cell Reports 2015

Noelle E Huskey, Tingxia Guo, Kimberley J Evason, Olga Momcilovic, David Pardo, Katelyn J Creasman, Robert L Judson, Robert Blelloch, Sco...

Two miRNA Clusters Reveal Alternative Paths in Late-Stage Reprogramming

Cell Stem Cell 2014

Ronald J Parchem, Julia Ye, Robert L Judson, Marie F Larussa,Raga Krishnakumar, Amy Blelloch, Michael C Oldham, Robert Blelloch

MicroRNA-based discovery of barriers to dedifferentiation of fibroblasts to pluripotent stem cells

Nature Structural & Molecular Biology 2013

Robert L Judson, Tobias S Greve, Ronald J Parchem, Robert Blelloch

microRNA Control of Mouse and Human Pluripotent Stem Cell Behavior

Annual Review of Cell and Developmental Biology 2013

Tobias S Greve, Robert L Judson, Robert Blelloch

Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells

Nature Biotechnology 2011

Deepa Subramanyam, Samy Lamouille, Robert L Judson, Jason Y Liu, Nathan Bucay, Rik Derynck, Robert Blelloch

miR-380-5p represses p53 to control cellular survival and is associated with poor outcome in MYCN-amplified neuroblastoma

Nature Medicine 2010

Alexander Swarbrick, Susan L Woods, Alexander Shaw, Asha Balakrishnan, Yuwei Phua, Akira Nguyen, Yvan Chanthery, Lionel Lim, Lesley J Ash...

Opposing microRNA families regulate self-renewal in mouse embryonic stem cells

Nature 2010

Collin Melton, Robert L Judson, Robert Blelloch

Embryonic stem cell-specific microRNAs promote induced pluripotency

Nature Biotechnology 2009

Robert L Judson, Joshua E Babiarz, Monica Venere, Robert Blelloch

The GP(Y/F) domain of TF1 integrase multimerizes when present in a fragment, and substitutions in this domain reduce enzymatic activity of the full-length protein

Journal of Biological Chemistry 2008

Hirotaka Ebina, Atreyi Ghatak Chatterjee, Robert L Judson, Henry L Levin

The self primer of the long terminal repeat retrotransposon Tf1 is not removed during reverse transcription.

Journal of Virology 2006

Angela Atwood-Moore, Kenneth Yan, Robert L Judson, Henry L Levin

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